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401 - Unauthorized: Access is denied due to invalid credentials

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I am getting error "401

I am getting Error "401 - Unauthorized: Access is denied due to invalid credentials.:" on a WCF service.   Following are the Authentication settings -

Anonymous Authentication-- Enabled

ASP.Net Impersonation   -- Enabled

Windows Authentication -- Disbaled

I have provided acccess to the IUSR account to the Application folder by adding the account to a group and then applying the group to the folder with Read and Excute permission. If I give direct access to IUSR account to the application folder, I get proper response and no error. In order to provide access to IUSR account to the application folder I have to use a group. I have also tried adding the IUSR id to IIS_IUSRS groups and then provide access to IIS_IUSRS group to folder but got same error.

Can someone please help me in resolving this error.  Thanks a lot in advance!!!

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